Fig. 2. Effects of SF-MSCs-CM on TGF-β-induced NRK-52E fibrosis at different time points. (A) The expression of α-SMA and Gal-3 in NRK-52E cells induced by TGF-β1 was detected by Western Blot after SF-MSCs-CM treatment. (B) Quantification of α-SMA. (C) Quantification of Gal-3. Results were normalized relative to the expression of GAPDH. N=3 (per group). Data are presented as mean±SD and analyzed by two-way ANOVA followed by Tukey post hoc testing. *p<0.05, vs. control group, ^#p<0.05, vs. TGF-β1＋SF-MSCs-CM (24 h) group, ^●p<0.05, vs. TGF-β1＋SF-MSCs-CM (48 h) group, ^▲p<0.05, vs. TGF-β1 ＋SF-DMEM (24 h) group, ^◆p<0.05, vs. TGF-β1＋SF-DMEM (48 h) group.

International Journal of Stem Cells 2023;16:52-65 https://doi.org/10.15283/ijsc22014

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