Fig. 4. Effects of SF-MSCs-CM on TGF-β1-induced NRK-52E cell autophagy at different time points. SF-MSCs-CM treatment upregulated the ratio of LC3B-II/I compared with the TGF-β1 group, accompanied by down-regulation of P62, SF-DMEM treatment after TGF-β1 treatment significantly downregulate the ratio of LC3B-II/I meanwhile upregulated the expression of P62 compared with the TGF-β1＋SF-MSCs-CM group. However, the ratio of LC3B-II/I of SF-MSCs-CM treatment for 48 h after TGF-β1 treatment could have a downward trend compared with 24 h, P62 does the opposite. (A) Quantification of LC3B-II/I. (B) Quantification of P62. Results were normalized relative to the expression of GAPDH. N=3 (per group). Data are presented as mean±SD and analyzed by two-way ANOVA followed by Tukey post hoc testing. *p<0.05, vs. normal group, ^#p<0.05, vs. TGF-β1＋SF-MSCs-CM group (24 h), ^●p<0.05, vs. SF-MSCs-CM group (48 h), ^▲p<0.05, vs. TGF-β1＋SF-DMEM group (24 h), ^◆p<0.05, vs. TGF-β1＋SF-DMEM group (48 h), ^&p<0.05, vs. SF-MSCs-CM group (24 h).

International Journal of Stem Cells 2023;16:52-65 https://doi.org/10.15283/ijsc22014

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