Fig. 6. Effects of SF-MSCs-CM on TGF-β 1-induced Gal-3 KD NRK-52E cells and Gal-3 OE NRK-52E cells fibrosis. The black and white bands illustrating the indicators identified through Western Blot (A), and statistical analysis of the ratio relative to GAPDH, including α-MSA (B), the ratio of TIMP1/MMP9 (C), FN (D), E-Cadherin (E), KIM-1 (F), Galectin-3 (G), the ratio of P-Akt/Akt (H) and P-GSK3β/GSK3β1 (I), and Snail (J). SF-MSCs-CM treatment significantly downregulated these indexes more surely than SF-DMEM treatment after TGF-β 1 treatment, except the expression of E-cad, which was upregulated. In the Gal-3 OE group, the indexes presented similar trends to those of the Gal-3 KD group, but higher than those of the same subgroups in the Gal-3 KD group. Results were normalized relative to the expression of GAPDH. N=3 (per group). Data are presented as mean±SD and analyzed by two-way ANOVA followed by Tukey post hoc testing. *p<0.05, vs. control group, ^★p<0.05, vs. TGF-β 1＋SF-MSCs-CM group, ^▲p<0.05, vs. TGF-β 1＋SF-DMEM group, ^●p<0.05, vs. SF-MSCs-CM group; compared Gal-3 KD NRK-52E cells with Gal-3 OE NRK-52E cells, ^#p<0.05, vs. normal group, ^@p<0.05, vs. TGF-β 1 group, &p<0.05, vs. TGF-β 1＋SF-MSCs-CM group, ^◆p<0.05, vs. TGF-β 1＋SF-DMEM group, ^p<0.05, vs. SF-MSCs-CM group.

International Journal of Stem Cells 2023;16:52-65 https://doi.org/10.15283/ijsc22014

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