Fig. 3. MiR-150-5p expressed in the MSCs-Exos effectively improves H₂O₂-treated HaCaT cell viability. (A) Detection of miR-150-5p expression in HaCaT cells treated with H₂O₂ by RT-qPCR. (B) Detection of miR-150-5p expression in MSC culture medium supernatants after exosome isolation and exosomes solution by RT-qPCR. (C) Detection of miR-150-5p expression in exosomes after digestion with RNase by RT-qPCR. (D) Fluorescent images of lentivirus-infected cells. (E) Verification of the overexpression and inhibition efficacy of miR-150-5p by RT-qPCR. (F) Detection of the viability of HaCaT cells in each group by the CCK-8 method. (G) Determination of the migration of HaCaT cells in each group by the plate scratch test. (H) Detection of the apoptosis of HaCaT cells in each group by flow cytometry. *p<0.05, **p<0.01, ***p<0.001, n=3. The data were presented in the form of mean±standard deviation. The comparison among multiple groups was performed by one-way analysis of variance. Tukey’s multiple tests were used for the post hoc comparison. MSC: mesenchymal stem cells, Exos: exosomes.

International Journal of Stem Cells 2022;15:359-71 https://doi.org/10.15283/ijsc21135

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