Fig. 4. MiR-150-5p regulates PI3K/AKT pathway by mediating PTEN. (A) Prediction of the binding site between miR-150-5p and PTEN by starBase (http://starbase.sysu.edu.cn/). (B, C) The detection of the expression level of PTEN in HaCaT cells treated with different concentrations of H₂O₂ by RT-qPCR (B) and western blot analysis (C). (D, E) Verification of the interaction between miR-150-5p and PTEN by dual-luciferase reporter assay (D) and RIP test (E). (F) The detections of miR-150-5p expression and PTEN mRNA expression in HaCaT cells transfected with miR-150-5p mimic, miR-150-5p inhibitor, or miR-150-5p inhibitor＋sh-PTEN by RT-qPCR. (G) PTEN, PI3K, and AKT protein expression and the phosphorylation of PI3K and AKT in HaCaT cells transfected with miR-150-5p mimic, miR-150-5p inhibitor, or miR-150-5p inhibitor＋sh-PTEN by western blot analysis. *p<0.05, **p<0.01, ***p<0.001, n=3. The data were presented in the form of mean±standard deviation. The comparison among multiple groups was performed by one-way analysis of variance. Tukey’s multiple tests were used for the post hoc comparison. MSC: mesenchymal stem cells, Exos: exosomes.

International Journal of Stem Cells 2022;15:359-71 https://doi.org/10.15283/ijsc21135

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