International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

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Fig. 3. In hBMSCs, miR-29a-3p negatively regulates the expression of FOXO3. (A) Bioinformatics analysis revealed that the FOXO3 3’UTR contained a potential binding site complementary to miR-29a-3p. (B) Dual-luciferase reporter gene assay confirmed that miR-29a-3p could directly bind to FOXO3 3’UTR. (C, D) qRT-PCR and western blot assays were performed to detect the expression of FOXO3 mRNA and protein in hBMSCs transfected with miR-29a-3p mimics or miR-29a-3p inhibitors. *p<0.01, **p<0.001.
International Journal of Stem Cells 2022;15:324-33 https://doi.org/10.15283/ijsc21147
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