International Journal of Stem Cells : eISSN 2005-5447

Fig. 5.

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Fig. 5. miR-29a-3p regulates cell proliferation and osteogenic differentiation of hBMSCs through Wnt/β-catenin signaling. (A, B) Western blot assay was used to detect β-catenin protein expression in hBMSCs transfected with miR-29a-3p mimics or miR-29a-3p inhibitors. (C) Immunofluorescence assay was used to detect the subcellular expression of β-catenin. (D∼G) qRT-PCR and Western blot assay was used to detect Runx2, ALP and OCN mRNA and protein expression, markers of osteogenic differentiation, in hBMSCs transfected with miR-29a-3p inhibitors and treated with XAV939. (H) CCK-8 assay was used to detect proliferation in hBMSCs transfected with miR-29a-3p inhibitors and treated with XAV939. (I) The ALP activity of hBMSCs transfected with miR-29a-3p inhibitors and treated with XAV939 was detected.
(J, K) Flow cytometry was used to detect the apoptosis and cell cycle of hBMSCs transfected with miR-29a-3p inhibitors and treated with XAV939. *p<0.05, **p<0.01, **p<0.001.
International Journal of Stem Cells 2022;15:324-33 https://doi.org/10.15283/ijsc21147
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