Fig. 1. Characterization of human dental pulp stem cells (DPSCs). (A) Primary DPSCs showed typical fibroblast or spindle morphology. (B) DPSCs were fused and grown in a swirl on the 13th day of primary culture (Scale bar=50 μm). (C) Control group for alizarin red S staining. (D) Alizarin red S staining of mineralized nodules after osteogenic-induced culture (Scale bar=200 μm). (E) Control group for oil red O staining. (F) Oil red O positive staining after adipogenic differentiation (Scale bar=200 μm). (G) Flow cytometry assay showing DPSCs with positive expression for CD44, CD73, CD90 and CD105, and negative expression CD34, CD11b, CD19, CD45 and HLA-DR.

International Journal of Stem Cells 2022;15:384-94 https://doi.org/10.15283/ijsc21240

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