International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

Download original image
Fig. 3. NBCe1 inhibition promoted the odontogenic differentiation of DPSCs. (A) Real-time PCR indicated higher expressions of odontogenic genes (DMP1, DSPP, Runx2, OCN and OPN) in the MM+S0859 group compared to the NC or MM groups (**p<0.01; *p<0.05). (B) Alizarin red staining showed obvious mineralized nodules in the MM+S0859 group compared to the control group (Scale bar=50 μm). (C) A CPC assay quantified the mineralized nodules and revealed that the concentration of mineralized nodules was higher in the MM+S0859 group compared to the MM group (***p<0.001, *p<0.05). (D) Protein expression levels of p65 and p-p65. (E) The ratio of p-p65/p65 in the S0859-treated group was higher compared to the NC and MM group (*p<0.05, **p<0.01). NC: control medium, MM: mineralized-inducing medium, CPC: cetylpyridinium chloride.
International Journal of Stem Cells 2022;15:384-94 https://doi.org/10.15283/ijsc21240
© 2022 International Journal of Stem Cells