International Journal of Stem Cells : eISSN 2005-5447

Fig. 4.

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Fig. 4. Effect of NF-κB inhibitor on the odontogenic differentiation of DPSCs treated with S0859. (A) A Real-time PCR assay demonstrated that the mRNA expression levels of DSPP, DMP1, Runx2, OCN, and OPN in the S0859+PDTC group were decreased compared to that in the S0859 group (*p<0.05, **p<0.01). (B) Alizarin red staining of mineralized nodules was more obvious in the S0859 group than in the S0859+PDTC group (Scale bar=50 μm). (C) CPC quantification showed that the concentration of mineralized nodules in the S0859 group was higher than in the S0859+PDTC group (*p<0.05, **p<0.01). (D) Protein levels of p65 and p-p65 in the MM group, MM+S0859 group, MM+PDTC group, and MM+S0859+PDTC group. (E) Quantification of the p-p65/p65 ratios after inhibition of the NF-κB pathway (*p<0.05, **p<0.01). NC: control medium, MM: mineralized-inducing medium, CPC: cetylpyridinium chloride.
International Journal of Stem Cells 2022;15:384-94 https://doi.org/10.15283/ijsc21240
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