International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. Induction of posterior DE from miPSCs. (A) A schematic of the differentiation trajectory and the markers expressed at each stage of differentiation. (B) Expression analyses of CDX2, SOX17, and FOXA2 in the cells by qRT-PCR on day 3 (mean±SD for six independent experiments, ****p<0.0001). (C) Western blot analysis of CDX2 in miPSCs, miPSC-derived urothelial cells, mouse urothelial cells and miPSCs at day 3 after induction of differentiation. Scale bar, 130 μm (n=5). (D) Western blot analysis of CDX2 in miPSCs and differentiated miPSCs on day 3 (n=5). (E) Immunostaining of CDX2 in miPSCs, differentiated miPSCs on day 3 and mouse colon. Scale bar, 130 μm (these images are representative of four independent experiments). (F) Immunostaining of SOX17 and FOXA2 in differentiated miPSCs on day 3. Scale bar, 130 μm (these images are representative of four independent experiments). DE: definitive endoderm, miPSCs: mouse-induced pluripotent stem cells, SD: standard deviation, Uro: mouse urothelial cells.
International Journal of Stem Cells 2022;15:347-58 https://doi.org/10.15283/ijsc21250
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