Fig. 3. Effects of CHIR99021 treatment on posterior DE induction. (A) Expression analyses of CDX2, SOX17, and FOXA2 in differentiated cells treated with different doses of CHIR99021 by qRT-PCR (mean±SD for six independent experiments, ****p<0.0001). (B) Morphology of differentiated cells after treatment with 4 μM, 6 μM, or 8 μM CHIR99021 for 3 days. Scale bar, 130 μm. (C) Expression analyses of CDX2 SOX17, and FOXA2 in differentiated cells that underwent different durations of CHIR99021 treatment by qRT-PCR (mean±SD for six independent experiments, ****p<0.0001). DE: definitive endoderm, SD: standard deviation.

International Journal of Stem Cells 2022;15:347-58 https://doi.org/10.15283/ijsc21250

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