Fig. 4. Induction of caudal hindgut from posterior DE. (A) Expression analyses of HOXA13 and HOXD13 in the cells by qRT-PCR on day 7 (mean±SD for six independent experiments, ****p<0.0001). (B) Im-munostaining of HOXD13 in miPSCs and differentiated miPSCs on day 7. Scale bar, 130 μm (these images are representative of four indepen-dent experiments). DE: definitive endoderm, miPSCs: mouse-induced pluripotent stem cells, SD: standard deviation.
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