International Journal of Stem Cells : eISSN 2005-5447

Fig. 5.

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Fig. 5. Induction of urothelial cells from caudal hindgut. (A) Expression analyses of Uroplakin IA, Uroplakin IB, Uroplakin II, Uroplakin III, CK20, CK5, CK7, CK13, ZO-1, and E-cadherin in the cells by qRT-PCR on day 16 (mean±SD for six independent experiments, ****p<0.0001). (B) Western blot analysis of Uroplakin Ib and Uroplakin III in miPSCs, miPSC-derived urothelial cells, mouse urothelial cells and mouse colon (n=5). (C) Western blot analysis of CK20, CK7, CK13, ZO-1, and E-cadherin in miPSCs, miPSC-derived urothelial cells and mouse urothelial cells (n=5). (D) Immunostaining of Uroplakin II, CK20, CK7, CK13, ZO-1, and E-cadherin in differentiated miPSCs on day 16. Scale bar, 130 μm (these images are representative of four independent experiments). (E) Morphology of cells at different stages of differentiation. Scale bar, 130 μm. miPSCs: mouse-induced pluripotent stem cells, SD: standard deviation, Uro: mouse urothelial cells.
International Journal of Stem Cells 2022;15:347-58 https://doi.org/10.15283/ijsc21250
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