Fig. 5.
Fig. 5. LIPUS promotes the expression of Piezo1, and GsMTx4 inhibits this effect. (A) Piezo1 miRNA expression of PDLSCs with endothelial induction and treatment with LIPUS (50 mW/cm2) for 14 days, **p<0.005. (B) Piezo1 miRNA expression of PDLSCs cocultured with different concentrations of GsMTx4 and after LIPUS (50 mW/cm2) treatment for 24 h, **p<0.005. (C) Western blot results and statistical results: Piezo1 expression of PDLSCs with endothelial induction and treatment with GsMTx4 and LIPUS (50 mW/cm2) for 14 days: V: endo-thelial induction in noninflammatory condition, VG: endothelial induction and cocultured with GsMTx4 in noninflammatory condition, *p< 0.05, ***p<0.0005. (D) Immunofluorescence staining results: Piezo1 expression of PDLSCs with endothelial induction and treatment with GsMTx4 and LIPUS (50 mW/cm2) in inflammatory or noninflammatory conditions for 14 days, magnification: 4×, a, b: Statistical analysis results: V: endothelial induction in noninflammatory conditions, VG: endothelial induction and coculture with GsMTx4 in noninflammatory conditions, LV: endothelial induction in inflammatory conditions, LVG: endothelial induction and coculture with GsMTx4 in inflammatory conditions, *p<0.05, ***p<0.0005.
International Journal of Stem Cells 2022;15:372-83 https://doi.org/10.15283/ijsc22024
© 2022 International Journal of Stem Cells
