Fig. 6. Piezo1 regulates the endothelial differentiation and angiogenesis of PDLSCs treated with LIPUS. (A) CD31 miRNA expression of PDLSCs treated with GsMTx4 with endothelial induction and LIPUS treatment (50 mW/cm²) under inflammatory or noninflammatory conditions for 14 days, **p<0.005, ***p<0.0005. a: noninflammatory conditions; b: inflammatory conditions. (B) Flow cytometric results of PDLSCs: CD31 expression with treatment with GsMTx4 under endothelial induction and LIPUS treatment (50 mW/cm²) under inflammatory or noninflam-matory conditions for 14 days: I: V: endothelial induction under noninflammatory conditions, II: VG: endothelial induction and coculture with GsMTx4 under noninflammatory conditions, III: LV: endothelial induction under inflammatory conditions, IV: LVG: endothelial induction and coculture with GsMTx4 under inflammatory conditions. (C) The tube formation results of PDLSCs containing GsMTx4 under endothelial induction and treatment with LIPUS (50 mW/cm²) under inflammatory or noninflammatory conditions: The grouping is the same as in Fig. 6B, magnification: 4×; a∼c: Statistical analysis, **p<0.005, ***p<0.0005. (D) Immunofluorescence staining results of PDLSCs: CD31 expression of PDLSCs with GsMTx4 under endothelial induction and treatment with LIPUS (50 mW/cm²) in inflammatory or noninflammatory conditions for 14 days. The grouping is the same as in Fig. 5D, magnification: 4×. a, b: Statistical results, **p<0.005, ***p<0.0005.

International Journal of Stem Cells 2022;15:372-83 https://doi.org/10.15283/ijsc22024

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