Fig. 3. Treatment with the ERK inhibitor U0126, led to an increase in osteogenesis in eADSCs and 3, 4’-DHF eADSCs via BMP signaling. (A) Western blot analysis of phosphorylated ERK in eADSCs and 3, 4’-DHF eADSCs in the presence or absence of U0126. (B) qRT-PCR of osteogenesis marker gene expression in eADSCs and 3, 4’-DHF eADSCs treated with U0126. (C) qRT-PCR analysis of BMP2 and BMP4 gene expression in the presence or absence of U0126 and PD98059 in eADSCs and 3, 4’-DHF eADSCs. Error bars represent±SD from at least three independent experiments (*p<0.05).

International Journal of Stem Cells 2022;15:334-45 https://doi.org/10.15283/ijsc22044

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