International Journal of Stem Cells : eISSN 2005-5447

Fig. 4.

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Fig. 4. 3, 4’-DHF regulates osteogenic differentiation by modulation of Reactive Oxygen Species (ROS) signaling. (A) Intracellular ROS level according to 2’, 7’-dichlorodihydrofluorescein diacetate (H2DCFDA) fluorescence by flow cytometry. (B) The intensity of H2DCFDA fluorescence in N-acetyl cysteine (NAC) treated eADSCs and 3, 4’-DHF eADSCs. (C) Expression of osteogenesis marker genes in NAC treated or untreated eADSCs and 3, 4’-DHF eADSCs according to qRT-PCR analysis. (D) Expression of ROS-related genes in eADSCs and 3, 4ʹ-DHF eADSCs in the presence or absence of NAC treatment. Error bars represent±SD from the mean of three independent experiments (*p<0.05).
International Journal of Stem Cells 2022;15:334-45 https://doi.org/10.15283/ijsc22044
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