International Journal of Stem Cells : eISSN 2005-5447

Fig. 5.

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Fig. 5. Co-treatment with 3, 4’-DHF, U0126, and NAC regulated osteogenic differentiation. (A) ROS levels were determined by measuring H2DCFDA fluorescence using a flow cytometer, with or without U0126 treatment in eADSCs or 3, 4’-DHF-treated eADSCs during osteogenic differentiation. (B) Intracellular ROS levels were measured following treatment with U0126 or NAC in eADSCs and 3, 4’-DHF eADSCs. (C) qRT-PCR analysis of osteogenic differentiation marker gene expressions in eADSCs and 3, 4’-DHF eADSCs, in the presence or absence with U0126 or NAC. Each experiment was repeated in triplicate and data are presented as means±SD (*p<0.05).
International Journal of Stem Cells 2022;15:334-45 https://doi.org/10.15283/ijsc22044
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