Fig. 2. Detection of morphology, iron content, and live cell ratio of SHED treated with various concentrations of MIRB (A). Morphology of SHED labeled with various concentrations of MIRB. SHED nuclei are stained with DAPI (blue); the cytoskeleton is stained with FITC-phalloidin (green); and MIRB particles are shown with Rhodamine B (red). (B) Prussian blue staining was used to detect Fe3+. (C) Iron content analysis of SHED. (D) Percentage of live cells was analyzed with Trypan blue staining. *p<0.05. The scale bar indicates 100 μm.
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