Fig. 3. Immunocytochemical analysis to determine the maintenance of neural stem cells derived from Dnmt knockout (KO) ESCs. After passage three, NSCs from three types of Dnmt KO ESCs, including Dnmt1 KO, Dnmt3a/3b double KO (Dnmt3 DKO), and Dnmt1/3a/3b triple KO (Dnmt TKO), were no longer immunoreactive to the neural stem cell markers Sox2 and Nestin. Control NSCs derived from Olig2-GFP ESCs maintained NSC marker ex-pression. Nuclei were counter-stained with Hoechst (blue); scale bars: 50 μm.

International Journal of Stem Cells 2023;16:44-51 https://doi.org/10.15283/ijsc22138

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