Fig. 4. Differentiation of Dnmt knockout (KO) NSCs into neural subtypes. (A) Immunocytochemical analysis of control (Olig2-GFP) and three types of Dnmt KO NSCs, including Dnmt1 KO, Dnmt3a/3b double KO (Dnmt3 DKO), and Dnmt1/3a/3b triple KO (Dnmt TKO). During the culture, Dnmt KO NSCs spontaneously differentiated into neurons (Tuj1＋), astrocytes (GFAP＋), and oligodendrocytes (O1＋). Nuclei were counter-stained with Hoechst (blue); scale bars: 200 μm for Tuj1 and GFAP, and 100 μm for O1. Control NSCs derived from Olig2-GFP ESCs maintained self-renewal and were not stained by antibodies for Tuj1, GFAP, and O1; scale bar: 200 μm. (B) Representative karyotype of control and three types of Dnmt KO cell lines. Karyotyping revealed no chromosome abnormalities in all groups; scale bar: 5 μm.

International Journal of Stem Cells 2023;16:44-51 https://doi.org/10.15283/ijsc22138

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