Fig. 3. γδ T cells from HE were successfully generated under defined culture conditions. (A) Representative morphology of γδ T cells from HE until day 39. Wright and Giemsa staining showed lymphoid lineage cells. Magnification 20×. (B) Flow cytometry results revealed that markers for γδ T cells were expressed in differentiated cells at days 26 and 30. The numbers of γδ T cells were significantly increased. Results are shown as the means±SEM from at least two independent experiments *p<0.05, **p<0.01. (C) Immunochemistry findings for CD56 and NKG2D expression in differentiated cells, including HE and floating cells, on day 26. Magnification 20×. (D) FACS results showed no difference in the percentages of Vδ2^＋CD3^＋ and Vγ9^＋CD3^＋ cells in HE and floating cells, respectively. Results are from at least three independent experiments. (E) FACS results revealed that the numbers of CD3, TCR Vδ2^＋, TCR Vγ9^＋, and Vδ2^＋Vγ9^＋ in CD3 cells gradually increased over time in the floating cells than those in HE. Results are shown as the means±SEM from at least two independent experiments. *p<0.05. (F) Genes according to each developing steps were determined in differentiated cells. mRNA expression of representative marker genes was determined through qRT-PCR. Results are shown as the means±SEM.

International Journal of Stem Cells 2023;16:108-16 https://doi.org/10.15283/ijsc22150

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