Fig. 1. Preparation of mRNA encoding EGFP. Prepared plasmid DNA of EGFP mRNA was linearized using the restriction enzyme EcoRV. Linearized DNA was used as a template for in vitro transcription. To prepare the final mRNA, a 5’-cap and poly(A) tail were added to the in vitro-transcribed RNA.

International Journal of Stem Cells 2023;16:117-22 https://doi.org/10.15283/ijsc22125

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