Fig. 1. LCLs are refractory to retroviral transduction. (A) Schematic representation of retroviral transduction procedure. (B) Quantification of iPSC colonies that had emerged from retroviral OSKM-transduced LCLs and fibroblasts. Data are presented as mean±SEM (n=3). (C) No GFP^＋ cells were observed in retroviral GFP-transduced LCLs, as determined by a fluorescent microscope. 30 μl and 50 μl of GFP virus were used for transduction. scale bar, 100 μm. (D) GFP^＋ cells were observed in retroviral GFP-transduced fibroblasts, as determined by a fluorescent microscope. 50 μl of GFP virus were used for transduction. scale bar, 100 μm. (E) Flow cytometry analysis of retroviral OSKM-transduced LCLs and fibroblasts. Non-infected cells were used for gating.

International Journal of Stem Cells 2023;16:36-43 https://doi.org/10.15283/ijsc22177

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