Fig. 2. LCLs are refractory to lentiviral transduction. (A) Schematic representation of lentiviral transduction procedure. (B) No tdTomato^＋ cells were observed in lentiviral OSKMT-transduced LCLs, as determined by a fluorescent microscope. 50 μl and 100 μl of tdTomato virus were used for transduction. scale bar, 100 μm. (C) tdTomato^＋ cells were observed in lentiviral OSKMT-transduced fibroblasts, as determined by a fluorescent microscope. 30 μl of GFP virus was used for transduction. scale bar, 100 μm. (D) Flow cytometry analysis of lentiviral OSKMT-transduced LCLs and fibroblasts. Non-infected cells were used for gating. (E) Quantification of iPSC colonies that had emerged from retroviral OSKM-transduced LCLs and fibroblasts. Data are presented as mean±SEM (n=3).

International Journal of Stem Cells 2023;16:36-43 https://doi.org/10.15283/ijsc22177

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