Fig. 4. Reprograming of LCLs into iPSCs by electroporation. (A) Schematic representation of reprogramming procedure. We electroporated LCLs with episomal vector encoding reprograming factors and cultured them in MEF-CM medium supplemented with sodium butyrate, SB431542 and SGC0946. These compounds were supplied to the medium for the first 14 days. (B) At one week post-transfection, small iPSC colonies were emerged in the plate. scale bar, 200 μm. (C) At three weeks post-transfection, the iPSC colonies appear large enough for picking. (D) Expression of pluripotency makers (SALL4, DPPA4, OCT4, and SSEA4) in iPSCs. DAPI was used for staining cell nuclei. scale bar, 100 μm. (E) iPSCs formed embryoid bodies. scale bar, 200 μm.

International Journal of Stem Cells 2023;16:36-43 https://doi.org/10.15283/ijsc22177

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