Fig. 2. Reconstruction of the developmental trajectory of BMMSCs and osteoblasts. (A) Cell differentiation pseudotime trajectory of osteogenesis clusters reconstructed with diffusion mapping. The trajectory graph on the upper right showed the direction of pseudotime from deep to shallow. (B) Distribution of each cell subpopulation along the pseudotime. (C) The expression levels (log-normalized) of key specific genes fluctuated with osteogenesis development. The x-axis indicated the pseudotime, while the y-axis represented the log-normalized gene expression levels. The colors corresponded to the six different osteogenesis cell subsets. (D) UMAP plots of osteogenesis cells, colored by expression of the indicated genes with roles in osteogenic differentiation.

International Journal of Stem Cells 2023;16:342-55 https://doi.org/10.15283/ijsc22101

© 2023 International Journal of Stem Cells