Fig. 1. Schematic representation of the protocol for the differentiation of hiPSC into CM. (A) In the XC culture condition, hiPSC-CM were generated on MatrigelTM-coated plates according to the modified GiAB (MM.1) protocol. Up to 5 days after the differentiation into CM commenced, 1~1.5 vol% MatrigelTM was added to the medium. (B) In the XF culture condition, hiPSC were differentiated into cardiac-lineage cells on VTN-N-coated 12-well culture plates using the PSC Cardiomyocyte Differentiation Kit.
© 2023 International Journal of Stem Cells