Fig. 2. Analysis of the characteristics of hiPSC-CM. (A) Microscopic image of cells of cardiac lineage at 1, 2, and 4 weeks after the differentiation of hiPSC into CM under XC and XF conditions. (B) mRNA expressions of cardiac lineage-specific marker genes in XC- and XF-hiPSC-CM were compared at 0, 2, and 4 weeks post-differentiation using reverse transcription-quantitative PCR. The expression values were normalized against that of 18S rRNA as a housekeeping gene. n=3 per group, *p<0.05, XC-hiPSC-CM vs. XF-hiPSC-CM using unpaired Student’s t-test. (C) Protein expression of cardiac lineage-specific markers was determined by immunostaining at 4 weeks after differentiation under the XF condition. MLC-2v, myosin light chain-2v (ventricular/cardiac muscle isoform) (red); cTnT, cardiac troponin T (green); α-SMA, α-smooth muscle actin (red); α-actinin, a cytoskeletal actin-binding protein (green). Cell nuclei were counterstained using DAPI (blue).

International Journal of Stem Cells 2023;16:281-92 https://doi.org/10.15283/ijsc22158

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