Fig. 4. Evaluation of immunogenic Neu5Gc in hiPSC-CM using an indirect ELISA. (A) Neu5Gc was only detected in XC complete media. (B) Neu5Gc expression in the XC-conditioned media and XC-hiPSC-CM lysates was significantly higher at 2 to 4 weeks of differentiation than at 0 weeks (hiPSC). Neu5Gc was not detected in XF media and XF-hiPSC-CM lysates. Fetal bovine serum was used as a positive control and experimental internal control. Statistical analysis was performed using analysis of variance, followed by Tukey’s multiple comparisons test. Values are presented in terms of means±SEM. **p<0.01, ***p<0.001. n=3 per group.

International Journal of Stem Cells 2023;16:281-92 https://doi.org/10.15283/ijsc22158

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