Fig. 3. Three patient-derived GSCs (GSC-01, GSC-02 and GSC-03) were transfected for 6 hours by siNC or siPTHrP. Then, knockdown PTHrP cells were exposed with 50 nM/ml rPTHrP or equal volume of the medium. (A) After culturing for 3 days, BrdU (10 mg/ml) incorporation assay was used for detecting proliferation cells. Scale bar=100 μm. (B) The value represents the mean±standard deviation of three independent experiments (n=3). *p<0.05 versus siNC group; ###p<0.001 versus siPTHrP group.
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