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List of primers and antibodies
| qPCR primers | ||
|---|---|---|
| Gene | Forward | Reverse |
| GGGGTTCTATTTGGGAAGGTAT | TGTTGTCAGCTTCCTCCACC | |
| CAGAAGGCCTCAGCACCTAC | ATTGGAAGGTTCCCAGTCGG | |
| CAGGCCTGCAGCGCCATGAGCAGCCCG | CTGGGGCGGATCCGGGACCTGTCACAC | |
| CTTCAAGCACCTGCAGATTC | AGACCTGGATTTCACCGTGT | |
| AGGACAAGTACCGCGTGGTCTA | CCTCTGAACGTATGGAGGAGGA | |
| GGTTGCACAAAAGTTTATAC | GGCTTGGCGCCCGTGTGCTTCC | |
| TCCTGGAATACCCCATACTTAGCA | GCCGCCGCAACTGTTG | |
| ACAGCGAAGGGAAATGTAAAAGC | GGGCCCCAGAGACGCTAA | |
| TCCTTGGCCGTCTACTGGAA | AGTGGATTAAACGCTAATTCAGTAATAC | |
| TGCAAAAAGCGACCTACTACGA | CGTCGGCGCCCAAG | |
| AGGTGCAGGCATCCAGGTACT | GGGTTGGCAGCCATGTCTAC | |
| TTACCCCTGGATGCAGCGAATG | CCGCGTTAGGTAGCGATTGAAG | |
| GTCGCCCACACAGCTCTTCCC | AATAGGAACTCCTTCTCCAGCTC | |
| CAGCAAGCACAAAGAGGAGAAGG | AGTTCCAGCGTCTGGTACTTGG | |
| TGTGTGAGCGCAGTCTCTACAGA | CGGCCTCAGGTTGGAGAAG | |
| AAATGTACTGCCCCAAAGAGCA | ATCCGAGGGATGGGAGACC | |
| CCACTGGCTGCTGGACTGTT | TATGACTGGGCCAGGTTCTTTG | |
| CTGGGCTACGGCTACCACTTC | GCGATGACTTGAGCGCATT | |
| CCACCTTCCCAGCTTGATT | TTAGCCAGCTGGTGGAGACT | |
| CCAGTGCCAGCCCAATAGAC | TGGACAATAGCAAAGCGGTTG | |
| ATGCTTTCCATCCACTCACC | TTCACTGCTCTCTCCCGTTT | |
| TCAGAAGGGTGATGAGTTTGTCC | GGGGCGCTTATGGTCCTTG | |
| CGGTTTTGAAGATGAACTCTCAG | GCTCTTCCACCGCCTCTT | |
| GCCTGTTCTACTGCAAGTTCCAC | CACAGATGACCACTTTGGTGGC | |
| GCCAAGAGAAAGGTTGTGAAGA | GTGTACATGCTGCTCTGGCG | |
| GGCCCTGTAATTGGAATGAG | GGCCCTGTAATTGGAATGAG | |
| Antibodies | ||
|---|---|---|
| Target | Company | Application |
| SOX17 | R&D system, AF1924 | Western blotting, Immunofluorescence |
| FOXA2 | Santa Cruz, sc-374376 | Western blotting, Immunofluorescence |
| CDX1 | Santa Cruz, sc-398470 | Western blotting, Immunofluorescence |
| KLF5 | NOVUS, NBP1-49538 | Western blotting, Immunofluorescence |
| SATB2 | Santa Cruz, sc-81376 | Western blotting, Immunofluorescence |
| MUC2 | Santa Cruz, sc-515032 | Immunofluorescence |
| MUC3 | Santa Cruz, sc-7315 | Western blotting, Immunofluorescence |
| MUC4 | Santa Cruz, sc-33654 | Western blotting, Immunofluorescence |
| EpCAM | eBioscience, 14-5791-81 | FACS, Immunofluorescence |
| CD133 | Santa Cruz, sc-365537 | Immunofluorescence |
| Villin | Santa Cruz, sc-58897 | Western blotting, Immunofluorescence |
| DEFA5 | NOVUS, NB110-60002 | Western blotting |
| CHGA | NOVUS, NBP2-29428 | Western blotting, Immunofluorescence |
| LGR5 | Abcam, ab75732 | Western blotting |
| TUJ1 | Biolegend, 802001 | Immunofluorescence |
| KI-67 | eBioscience, 14-5698-82 | Immunofluorescence |
| SOX9 | Cell signaling, 82630 | Immunofluorescence |
| Serotonin | Immunostar, 20080 | Immunofluorescence |
| P75 NGFR | Santa Cruz, sc-13577 | FACS |
| HNK-1 | Sigma Aldrich, C6680 | FACS |
| GAPDH | Santa Cruz, sc-47724 | Western blotting |
| Substance P | Abcam, ab288318 | ELISA |
| CHGA | R&D systems, DY9098-05 | ELISA |
| Inhihitors | ||
|---|---|---|
| Target | Company | Application |
| Vapreotide | MCE, HY-P3802 | NK1R antagonist |
| Spantide II | MCE, HY-P1722 | NK1R antagonist |