Fig. 5. Effects of indomethacin on ALP activity and mRNA levels of bone-related genes in DFATs treated with BMP9 and/or LIPUS. (A) The effect of indomethacin on ALP activity. (B∼D) The effect of indomethacin on expression of bone-related genes; (B) Runx2, (C) Osx, (D) Opn. DFATs were cultured with BMP9 (100 ng/ml) and indomethacin (1 μM) for 2 days (A∼C) and 6 days (D) in ODM. LIPUS treated cells were exposed to LIPUS for 20 minutes every day. Values are shown as mean±SD (n=4). **p<0.01.
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