Fig. 2. GRC assay for hUC-MSC using FreSHtracer and MitoFreSHtracer. (a∼c) FreSHtracer- or (d∼f) MitoFreSHtracer-loaded hUC-MSCs were treated with 0, 0.125, 0.25, 0.5, and 1 mM of diamide (DA) or 0.1 mM N-ethylmaleimide (NEM), and analyzed using fluorescence confocal microscopy. (a, b) Representative images of confocal microscopy are shown with ×100 magnification. Scale bars=100 μm. (c, d) Kinetics of the FR levels (F510/F580 ratio) were plotted over time. (e, f) The GRC levels were calculated. Error bars represent means±SEM. n.s.: not significant, #: control group. *p<0.05, **p<0.01, ***p<0.001.

International Journal of Stem Cells 2023;16:356-62 https://doi.org/10.15283/ijsc23047

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