Fig. 4. Comparing the GRC data of hBM-MSCs with different passages. hBM-MSCs passaged to P4 and P9 were loaded with (a∼c) FreSHtracer or (d∼f) MitoFreSHtracer, treated with 0, 0.06, 0.12, 0.3, 0.6, and 0.9 mM of diamide (DA) or 0.1 mM N-ethylmaleimide (NEM), and were analyzed using fluorescence confocal microscopy. The FR (F510/F580 ratio) levels were plotted over time in (a, d) P4 and (d, e) P9 hBM-MSCs, respectively. The GRC and mGRC levels of both cells loaded (c) FreSHtracer and (f) MitoFreSHtracer, respectively were calculated based on the FR kinetics. Error bars represent means±SEM. #: control group. *p<0.05, **p<0.001, ***p<0.001.

International Journal of Stem Cells 2023;16:356-62 https://doi.org/10.15283/ijsc23047

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