Fig. 2. Generation of neural precu-rsor cells (NPCs) from human pluripotent stem cells (hPSCs). (A) Sche-matic describing the differentiation protocol of NPCs via spherical neural mass (SNM) from hPSCs. hPSCs transitioned through stages of embryoid body (EB), neural rosette, SNMs, and NPCs. (B-E) Typical morphology of the hPSC colony. (F-I) hPSCs were indu-ced for 5 days with suspended EB. (J-M) After EB attachment, neural rosettes were induced for 5∼10 days. (N-Q) After the selection of the SNMs, they were passaged 3 times for purification. (R-U) After 3∼4 passages, the SNMs were dissociated into single cells. Scale bar=100 μm. hESC: human embryonic stem cell, hiPSC: human indu-ced pluripotent stem cell, Homo-SCNT-hESC: homozygous somatic cell nuclear transfer-hESC.