Examples of gene regulation using CRISPRa/i system
| CRISPR system | Delivery method | Type of cells | Target | Reference | ||
|---|---|---|---|---|---|---|
| Strategy | CRISPR type | Gene | Differentiation | |||
| CRISPRa CRISPRi | dCas9-VP64 dCas9-KRAB | Lentivirus | ESCs | SOX17 |
- | (79) |
| CRISPRa | dCas9-SAM | Lentivirus | MSCs | PPARG, CEBPAP PARG, CEBPA, PRDM16 | White adipocyte-like cells Beige adipocyte-like cells | (111) |
| CRISPRa | dCas9-VP64 dCas9-VPR | Lentivirus | iPSCs | Neuronal cells | (68) | |
CRISPRa/i: clustered regularly interspaced short palindromic repeats activation/interference, ESCs: embryonic stem cells, MSCs: mesenchymal stem cells, iPSCs: induced pluripotent stem cells.