Fig. 4. ML-130 affected the bio-habits of ectopic mesenchymal stem cells (ect-MSCs). ect-MSCs were stimulated by different concentrations of ML-130 (0, 0.28, and 0.56 μM) at different time points. (A) Colony-forming units (CFUs) decreased with the increase of ML-130 concentration at 48 hours (left panels). The proliferative effects of various concentration gradient of ML-130 on ect-MSCs (n=3) at 48 hours (right panel). (B) The invasion and migration capacities of ect-MSCs were significantly inhibited by the ML-130 at 0.28 and 0.56 μM (n=4) (left panels 200×, the number of invasion and migration cells per field); The transcription (C) and secretion levels (D) of interleukin (IL)-8 of ect-MSCs pretreated with ML-130 (n=4 in each group). **p<0.01.