Primer sequences for RT-PCR and qRT-PCR analysis
| Name | Direction | Primer sequence (5’-3’) | Target size (bp) |
|---|---|---|---|
| Human OCT4 | Forward | ACCCCTGGTGCCGTGAA | 190 |
| Reverse | GGCTGAATACCTTCCCAAATA | ||
| Human SOX2 | Forward | CAGCGCATGGACAGTTAC | 321 |
| Reverse | GGAGTGGGAGGAAGAGGT | ||
| Human NANOG | Forward | AAAGGCAAACAACCCACT | 270 |
| Reverse | GCTATTCTTCGGCCAGTT | ||
| Human LIN28 | Forward | CTTCGGCTTCCTGTCCAT | 122 |
| Reverse | CTGCCTCACCCTCCTTCA | ||
| Human RUNX2 | Forward | GTGCCTAGGCGCATTTCA | 78 |
| Reverse | GCTCTTCTTACTGAGAGTGGAAGG | ||
| Human COL1A1 | Forward | TCTGCGACAACGGCAAGGTG | 146 |
| Reverse | GACGCCGGTGGTTTCTTGGT | ||
| Human OCN | Forward | CGCTACCTGTATCAATGGCTGG | 123 |
| Reverse | CTCCTGAAAGCCGATGTGGTCA | ||
| Human ALP | Forward | GACCCTTGACCCCCACAAT | 68 |
| Reverse | GCTCGTACTGCATGTCCCCT | ||
| Human RANKL | Forward | ACATATCGTTGGATCACAGCACAT | 100 |
| Reverse | CAAAGGCTGAGCTTCAAGCTT | ||
| Human OPG | Forward | TGCTGTTCCTACAAAGTTTACG | 433 |
| Reverse | CTTTGAGTGCTTTAGTGCGTG | ||
| Human TNFα | Forward | GAGGCCAAGCCCTGGTATG | 91 |
| Reverse | CGGGCCGATTGATCTCAGC | ||
| Human GAPDH | Forward | CTGTTGCTGTAGCCAAATTCGT | 101 |
| Reverse | ACCCACTCCTCCACCTTTGA |
RT-PCR: real-time polymerase chain reaction, qRT-PCR: quantitative RT-PCR.