International Journal of Stem Cells : eISSN 2005-5447

Table. 1.

Table. 1.

Checklist for quality assessment of brain organoids

1. Essential cellular components
Neural stem cell-derived cells, such as neural progenitor cells (Nestin+ Sox2+), neurons (Pan-neuronal marker: TuJ1, NeuN), astrocytes, oligodendrocytes, and ependymal cells 95% or more
Optional: non-neural stem cell-derived cells in the brain, such as endothelial cells, pericytes, and microglia
Recommended testing methods: immunostaining or flow cytometry

2. Neural activity

The proportion of the brain organoid comprised of neurons, or the extent of the response at all electrodes using MEA or voltage sensors 10% or more
Recommended testing methods: Ca2+ imaging, MEA, voltage sensors, etc.

3. Gene expression

The organoid should show similarity with a Pearson’s correlation coefficient of 0.4 or more with at least one type of transcriptome profile for actual tissue from a brain region at a given developmental stage, based on bulk RNA-seq 0.4 or more
Recommended testing methods: comparison with region-specific RNA-seq data sets from the Allen Brain Atlas

4. Detection of neurotransmitters

Neurotransmitters should be detected in brain organoid lysate
Cerebrum: glutamate, GABA
Midbrain: glutamate, GABA, dopamine, serotonin
Spinal cord: glutamate, GABA, acetylcholine
Recommended testing methods: ELISA,HPLC, Raman spectrometry, LC-Mass, etc.

MEA: microelectrode array.

International Journal of Stem Cells 2024;17:158-81 https://doi.org/10.15283/ijsc24056
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