List of primers used for qPCR and ChIP assays in this study
Primer | 5’-sequence-3’ | Usage |
---|---|---|
mUsp7-F | CCTTAGCCCTCCGTGTTTTGT | qPCR |
mUsp7-R | CCAGTCGTTTTCCTTGTGGAAG | qPCR |
mAscl1-F | AGATGAGCAAGGTGGAGACG | qPCR |
mAscl1-R | TGGAGTAGTTGGGGGAGATG | qPCR |
mNgn1-F | ATGCCTGCCCCTTTGGAGAC | qPCR |
mNgn1-R | TGCATGCGGTTGCGCTCGC | qPCR |
mNeuroD1-F | CATGCCCCCGCATCTGCCAA | qPCR |
mNeuroD1-R | GCCATTGATGCTGAGCGGCG | qPCR |
mMap2-F | AAACAGGCGAAGGATAAAGT | qPCR |
mMap2-R | TGTTGTCAGTTGATCCGATT | qPCR |
mβIII-tubulin-F | AAGGTAGCCGTGTGTGACATC | qPCR |
mβIII-tubulin-R | ACCAGGTCATTCATGTTGCTC | qPCR |
mOlig-F | CACAGGAGGGACTGTGTCCT | qPCR |
mOlig-R | GGTGCTGGAGGAAGATGACT | qPCR |
mSox10-F | GTCAACGGTGCCAGCAAGAG | qPCR |
mSox10-R | TCAATGAAGGGGCGCTTGTC | qPCR |
mO4-F | CATCCGTCACAACCTGTCCT | qPCR |
mO4-R | GGCTTCTTCTTGGGGCCTTT | qPCR |
mGFAP-F | AGCGGCAAATGCGCGAACAG | qPCR |
mGFAP-R | GTGCTTTTGCCCCCTCGGAT | qPCR |
mAldh1L1-F | GAGCCACCTATGAGGGCATT | qPCR |
mAldh1L1-R | AAGAGGATGAGTCCCGCTTT | qPCR |
mS100β-F | CTGATCGCCTACACCCTTCC | qPCR |
mS100β-R | AAAGGAGAAGTCTGCCGAGC | qPCR |
mActin-β-F | GGACCTGACAGACTACCTCA | qPCR |
mActin-β-R | GTTGCCAATAGTGATGACCT | qPCR |
mOlig2-F | TGCTTATTACAGACCGAGCCAAC | ChIP |
mOlig2-R | CTAAATCCTAGCCACTTTGGAGAAGT | ChIP |
mGFAP-F | CGAGTGACTCACCTTGGCATAG | ChIP |
mGFAP-R | CCAGGATGCCAGGATGTCAG | ChIP |
mPax6-F | CACCAGACTCACCTGACACC | ChIP |
mPax6-R | GAACACACAGGTTGCACGTC | ChIP |
mActin-β-F | CCGTAAAGACCTCTATGCCAACAC | ChIP |
mActin-β-R | GCTAGGAGCCAGAGCAGTAATCTC | ChIP |
qPCR: quantitative polymerase chain reaction, ChIP: chromatin immunoprecipitation.