Table. 2.

List of primers used for qPCR and ChIP assays in this study

Primer 5’-sequence-3’ Usage
mUsp7-F CCTTAGCCCTCCGTGTTTTGT qPCR
mUsp7-R CCAGTCGTTTTCCTTGTGGAAG qPCR
mAscl1-F AGATGAGCAAGGTGGAGACG qPCR
mAscl1-R TGGAGTAGTTGGGGGAGATG qPCR
mNgn1-F ATGCCTGCCCCTTTGGAGAC qPCR
mNgn1-R TGCATGCGGTTGCGCTCGC qPCR
mNeuroD1-F CATGCCCCCGCATCTGCCAA qPCR
mNeuroD1-R GCCATTGATGCTGAGCGGCG qPCR
mMap2-F AAACAGGCGAAGGATAAAGT qPCR
mMap2-R TGTTGTCAGTTGATCCGATT qPCR
mβIII-tubulin-F AAGGTAGCCGTGTGTGACATC qPCR
mβIII-tubulin-R ACCAGGTCATTCATGTTGCTC qPCR
mOlig-F CACAGGAGGGACTGTGTCCT qPCR
mOlig-R GGTGCTGGAGGAAGATGACT qPCR
mSox10-F GTCAACGGTGCCAGCAAGAG qPCR
mSox10-R TCAATGAAGGGGCGCTTGTC qPCR
mO4-F CATCCGTCACAACCTGTCCT qPCR
mO4-R GGCTTCTTCTTGGGGCCTTT qPCR
mGFAP-F AGCGGCAAATGCGCGAACAG qPCR
mGFAP-R GTGCTTTTGCCCCCTCGGAT qPCR
mAldh1L1-F GAGCCACCTATGAGGGCATT qPCR
mAldh1L1-R AAGAGGATGAGTCCCGCTTT qPCR
mS100β-F CTGATCGCCTACACCCTTCC qPCR
mS100β-R AAAGGAGAAGTCTGCCGAGC qPCR
mActin-β-F GGACCTGACAGACTACCTCA qPCR
mActin-β-R GTTGCCAATAGTGATGACCT qPCR
mOlig2-F TGCTTATTACAGACCGAGCCAAC ChIP
mOlig2-R CTAAATCCTAGCCACTTTGGAGAAGT ChIP
mGFAP-F CGAGTGACTCACCTTGGCATAG ChIP
mGFAP-R CCAGGATGCCAGGATGTCAG ChIP
mPax6-F CACCAGACTCACCTGACACC ChIP
mPax6-R GAACACACAGGTTGCACGTC ChIP
mActin-β-F CCGTAAAGACCTCTATGCCAACAC ChIP
mActin-β-R GCTAGGAGCCAGAGCAGTAATCTC ChIP

qPCR: quantitative polymerase chain reaction, ChIP: chromatin immunoprecipitation.

International Journal of Stem Cells 2024;17:427-36 https://doi.org/10.15283/ijsc23202
© 2024 International Journal of Stem Cells