Fig. 3. The expression of transcription factors related to mouse neurogenesis upon Usp7 knockout (KO). (A-C) Relative expression levels of oligodendrocyte-specific transcription factors (A) Olig2, (B) Sox10, and (C) O4 messe-nger RNA (mRNA) were analyzed by real-time polymerase chain reaction (PCR). Samples were collected on day 9 after differentiation from control and Usp7 KO P19 cells. Results were normalized with the level of GAPDH. (D-F) Relative expression of astrocyte-specific transcription factor (D) GFAP, (E) ALDH1L1, and (F) S100β were analyzed by real-time PCR. Error bars were derived from three independent experiments. N=3. ***p<0.001.