Fig. 3. Immunofluorescence staining of three-dimensional explant culture of human dental pulp tissue. After 2-week culture, dental pulp explants were subjected to cryosectioning and immunostaining analysis. Fluorescence images with antibodies against hematopoietic marker (CD45), endothelial marker (CD31), stem cell marker (Nestin) and odontoblast markers (PHEX, Osterix, DMP1) are shown along with overlay of phase contract images. Nucleus was stained with 4’,6-diamidino-2-phenylindole (DAPI). Scale bars are indicated in the figure. Od: odontoblast arear, P: pulp area.