Fig. 4. Dual immunofluorescence staining of three-dimensional explant culture of human dental pulp tissue. (A) 2-week culture of dental pulp explant was subjected to cryosectioning and immunostaining with antibodies against Nestin (green) and LGR5 (red) along with overlay of phase contrast image. Nucleus was stained with 4’,6-diamidino-2-phenylindole (DAPI). Scale bar=200 μm. O: odontoblast arear, P: pulp area, BF: bright field. (B) Nestin-positive, LGR5-positive, and double-positive cells among DAPI-positive cells were quantified.