Fig. 3. Effects of L-type calcium channel on the odontogenic differentiation of Schwann cells (SCs) in vitro. (A) Immunofluorescence staining of DSP in SCs after odontogenic differentiation. Scale bar=50 μm. (B) The mRNA expression of DSPP, DMP-1 and Runx2 in the three groups was measured at 14 days by real-time polymerase chain reaction. (i) Control: SCs in Schwann cell medium. (ii) OD: SCs in odontogenic differentiation medium (ODM). (iii) OD/Nimo(＋): SCs in ODM supplemented with 1μM nimodipine. Error bar: ±SD (n=3); *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 OD group with or without nimodipine versus control. (C) Images of Alizarin Red S staining at 28 days of odontogenic differentiation.