List of oligos used for cloning
| Name | Sequence (5’ to 3’) | Purpose |
|---|---|---|
| caccGTGGAGGGAACACACTCACGTgtttt | Golden Gate assembly cloning into pU6-tevopreq1-GG-acceptor plasmid | |
| ctctaaaacACGTGAGTGTGTTCCCTCCAC | ||
| epegRNA scaffold_F | AGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCG | |
| epegRNA scaffold_R | GCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAG | |
| gtgcACTTGGCCTACaTGAGTGTGTTCCC | ||
| cgcgGGGAACACACTCAtGTAGGCCAAGT | ||
| hU6 promoter_F | GAGGGCCTATTTCCCATGATT | Sanger sequencing |