Table. 2.

List of PCR primers for Sanger sequencing

Name Sequence (5’ to 3’) Size (bp) Purpose
ENG_e3_F CTGCCTGTCTGGGTGGCACAACCT 269 gDNA PCR, Sanger sequencing
ENG_e3_R CAGTAGGGACCTCCCATGGCCAGA
ENG OT(1)_F GCAAACGCTGTCCCTATCCT 262 Off-target (1) Sanger sequencing
ENG OT(1)_R CTCTCCCACCAACCTGGAAC
ENG OT(2)_F CCCCAGAGAGGTGATCGAGA 333 Off-target (2) Sanger sequencing
ENG OT(2)_R CATGGCAGGGTTTAGCCTCA

gDNA PCR: genomic DNA polymerase chain reaction.

International Journal of Stem Cells 2024;17:397-406 https://doi.org/10.15283/ijsc24084
© 2024 International Journal of Stem Cells