Fig. 3. Isolation of transfected human induced pluripotent stem cells (hiPSCs) and bulk sequencing for evaluating editing efficiency. (A) After 24 hours of nucleofection for prime editing, GFP-expressing cells were sorted by FACS to increase editing efficiency. (B) Isolated GFP-expressing cells were analyzed to examine efficiency. Bulk genomic DNA was extracted from sorted cells and the target sequence was amplified by polymerase chain reaction (PCR). Gel-extracted PCR product was sequenced and analyzed by EditR. The asterisk mark indicates the target base pair.