Fig. 5. Endothelial organoid (EO) formation and proangiogenic marker analysis. (A) Differentiation schedule for EOs from isogenic control and hereditary hemorrhagic telangiectasia human induced pluripotent stem cell (HHT hiPSC) line. (B) mRNA expression levels of proangiogenic markers, including VEGFR2, ANGPT2, Tie2, and CD31, were analyzed by quantitative polymerase chain reaction. Statistical significance: *p<0.05, **p<0.01, ***p<0.001.